| Application |
Isolation of RNA, DNA, and protein |
| Selling unit |
10 Prep(s), 50 Prep(s), 250 Prep(s) |
| Target |
RNA |
| CE certified |
No, research use only |
| Technology |
Silica membrane technology |
| Brand |
NucleoSpin |
| Format |
Mini prep |
| Handling |
Centrifugation |
| Lysate clarification |
Centrifugation, NucleoSpin Filter |
| Automated use |
No |
| Sample material |
Animal tissue, Cells, Human tissue, Plant |
| Sample amount |
< 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 100 mg plant tissue |
| Fragment size |
RNA > 200 nt, DNA < 30 kbp, protein 15–300 kDa |
| Typical yield |
< 70 µg RNA, < 6 µg DNA, < 1200 µg Protein |
| Theoretical binding capacity |
200 µg |
| Typical purity A260/A280 |
RNA 1.9−2.1, DNA 1.7−1.9 |
| Typical RIN |
> 9 |
| Elution volume |
RNA 40–120 µL, DNA 100 µL, Protein 10–100 µL |
| Preparation time |
30 min/6 preps (RNA), 45 min/6 preps (RNA and DNA), + 35 min/6 preps (protein) |
| Typical downstream application |
Chip hybridization, qRT−PCR, RNA-Seq |
| Storage temperature |
15–25 °C / 59–77 °F |
| Shelf life (from production) |
24 Month(s) |
| Hazardous material |
Yes |