MSC Serum-free Medium MH01

MSC Serum-free Medium MH01

Catalog No. Product Name Specification
MH000-N011 MSC Serum-free Medium MH01 500ml
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The MSC Serum-free Medium MH01 is a serum-free, xeno-free medium specifically optimized for the isolation and expansion of human mesenchymal stem cells (hMSCs) under fully serum-free, phenol red-free, and xeno-free conditions. It supports primary MSC isolation using explant and enzymatic digestion methods and enables expansion over multiple passages, preserving the stability of cell surface markers.

●    Serum-free, xeno-free.

●    High primary yield: 10⁶–10⁷ cells from a 15 cm umbilical cord in 14 days.

●    High expansion: uniform morphology, 6–10x fold expansion in 72 hours, stable for 20 passages.

●    Ready-to-use: retains multilineage differentiation potential and CD markers.

●    Independently developed, stable supply, GMP.

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Fig1. A statistical graph of the MSC expansion fold during passage culture, tested over 20 continuous passages using MH01 and a competitor product. The sample consisted of cryopreserved umbilical cord cells, passaged at a density of 10,000 cells/cm², with a passage time of 72 hours.
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Fig2. A statistical graph of the MSC doubling time during passage culture, tested over 20 continuous passages using MH01 and a competitor product. The sample consisted of cryopreserved umbilical cord cells, passaged at a density of 10,000 cells/cm², with a passage time of 72 hours.
MSC Serum-free Medium MH01

Fig3. A statistical graph showing the change in cell diameter of MSCs during passage culture, tested over 20 continuous passages using MH01 and a competitor product. The sample consisted of cryopreserved umbilical cord cells, passaged at a density of 10,000 cells/cm², with a passage time of 72 hours.
MSC Serum-free Medium MH01

Fig 4. Representative images of MSCs during continuous 12-passage culture, tested using MH01. The sample consisted of cryopreserved umbilical cord cells, passaged at a density of 10,000 cells/cm², with a passage time of 72 hours.
MSC Serum-free Medium MH01

Fig 5.Representative flow cytometry images of MSCs at passage 5 (P5), tested using MH01. The sample consisted of cryopreserved umbilical cord cells, passaged at a density of 10,000 cells/cm², with a passage time of 72 hours.
MSC Serum-free Medium MH01

Fig 6. Immunosuppressive ability test of MSCs at passage 8 (P8), using MH01. The comparison was made between culture media containing 5% FBS and 5% hPL.
No. Type Documents
1 MSDS MSDS_MHN01X
2 Manual Manual_MHN01X
3 Flyer compressed-05-MSC_en