OptiVitro® T Cell Serum-free Medium (phenol red-free)

Catalog No. Product Name Specification
TE000-N031 OptiVitro® T Cell Serum-free Medium  (phenol red-free) 500ml(bottle)
TE000-N032 OptiVitro® T Cell Serum-free Medium  (phenol red-free) 1000ml(bottle)
TE000-N071 OptiVitro® T Cell Serum-free Medium  (phenol red-free) 1L(bag)
TE000-N072 OptiVitro® T Cell Serum-free Medium  (phenol red-free) 2L(bag)
TE000-N074 OptiVitro® T Cell Serum-free Medium  (phenol red-free) 5L(bag)

OptiVitro® T Cell Serum-free Medium (phenol red-free) is a serum-free and xeno-free medium designed for the in vitro expansion of human T lymphocytes.

●    Xeno-Free: Compositions do not contain animal-derived components, as well as phenol red, ,ensuring cell expansion under xeno-free conditions.

●    Options of complete cytokine kit: Amplification kit and basal medium as required.

●    Compatibility: Optimized for cultivation and expansion of various T cell types (CAR-T, TCR-T, γδT/CAR-γδT, TIL, etc.)

●    Efficient T Cell Expansion: Supports robust T cell expansion with high cell viability after 14 days of culture

●    Quality Control: Manufactured in compliance with GMP and ISO standards, convenient access to Drug Master File (DMF).

●    Available in both bottle and bag formats, facilitating scale-up production and clinical translation

Content And Storage • Basal Medium   (1000 mL): store at 2–8°C

• Supplement UE01(8 mL): store at  2–8°C

and protect from light

Shipping   Condition Basal:   <25℃

Supplement:<25℃

Form Liquid Cell Type T cells
Serum Level Serum-free Product Type Cell Culture Medium
For Use With (Application) Cell   and Gene Therapy Research, Development,

and Manufacturing

Sterility Sterile-filtered
OptiVitro® T Cell Serum-free Medium (phenol red-free)

Figure 1. The viability of T Cells cultured in OptiVitro® T-SFM and medium is better than medium 1.

T Cell viability was determined every 2-3 days throughout the culture in OptiVitro® T-SFM and medium and medium1. Data represents the mean of three replicates for each independent tests.

Figure 2. The expansion folds of T Cells cultured in OptiVitro® T-SFM is better than medium 1.

T Cell density was tested every 2-3 days throughout the culture in OptiVitro® T-SFM and medium 1, and the expansion folds were calculated. Data represents the mean of three replicates for each independent tests.

Figure 3. The distribution of T Cell populations cultured in OptiVitro® T Cell Serum-free Medium is similar to medium 1.

T Cell phenotypic characterization was performed in OptiVitro® T Cell Serum-free Medium and medium 1 on D13. Data represents the mean of three replicates for each independent tests.

Figure 4. T-cell transduction in healthy people cultured with OptiVitro® T Cell Serum-free Medium i and medium 1.

The transduction efficiency of D5, D7, D9 and D13 during cell culture was tested. Compared with medium 1, OptiVitro® T cell serum-free medium had higher transduction efficiency.

No. Type Documents
1 MSDS MSDS_T
2 Manual Manual_TEN03X
3 Flyer compressed-03-T_en